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Saaxiibada ku jira shaybaadhka inta badan miyay ku wareereen faraqa u dhexeeya tuubooyinka PCR, tuubooyinka EP, iyo tuubooyinka sideed-tube? Maanta waxaan soo bandhigayaa kala duwanaanshaha iyo sifooyinka saddexdan

2023-07-11

Saaxiibada shaybaadhka ku jira miyay inta badan ku wareersan yihiin faraqa u dhexeeyatuubada PCRs, tuubooyinka EP, iyo tuubooyinka sideed-tubo? Maanta waxaan soo bandhigayaa kala duwanaanshaha iyo sifooyinka saddexdan

1. tuubada PCR

tuubada PCRs ayaa inta badan loo isticmaalaa waxyaabaha la isticmaalo ee tijaabooyinka noolaha. Tusaale ahaan, tuubooyinka Cotaus®PCR waxaa inta badan loo isticmaalaa in lagu bixiyo weelasha PCR (polymerase chain reaction) tijaabooyinka, kuwaas oo lagu dabaqi karo isu beddelka, isku xigxiga, methylation, cloning molecular, muujinta hidda-wadaha, Genotyping, daawada, sayniska forensic-ka iyo qaybaha kale. Tuubbada PCR ee caadiga ah waxay ka kooban tahay jir tuubo iyo dabool, iyo jirka tuubada iyo daboolka ayaa isku xiran.

Qalabkii PCR ee ugu horreeyay ma lahayn dabool kulul. Inta lagu jiro habka PCR, dareeraha tuubada hoose ayaa u soo bixi doona xagga sare. Daboolka convex-ka ah (taas oo ah, wareegga sare) waxaa loogu talagalay in lagu fududeeyo uumiga dareeraha si uu isugu ururiyo oo hoos ugu qulqulo. Si kastaba ha ahaatee, qalabka PCR ee hadda jira asal ahaan waa nooc daboolan oo kulul. Heerkulka sare ee daboolka PCR waa sarreeyaa heerkulka hoosena waa hooseeyaa. Dareeraha hoose ma fududa in uu uumi ugu baxo xagga sare, sidaas darteed intooda badan waxay isticmaalaan daboolyo fidsan.

2. tuubada EP

Sababtoo ah tuubada centrifuge markii ugu horeysay waxaa ikhtiraacay oo ay soo saartay Eppendorf, waxaa sidoo kale loo yaqaannaa EP tube.

Farqiga ugu weyn ee u dhexeeyatuubada PCRs iyo tuubooyinka microcentrifuge waa in tuubooyinka microcentrifuge guud ahaan ay leeyihiin derbiyo tuubo dhumuc weyn si loo hubiyo shuruudaha centrifugation, halkatuubada PCRs waxay leeyihiin derbiyo tuubo dhuuban si loo hubiyo xawaaraha wareejinta kulaylka iyo isku midnimada. Sidaa darteed, labadaba laguma qasi karo codsiyo wax ku ool ah, sababtoo ah tuubooyinka PCR ee khafiifka ah ayaa laga yaabaa inay qarxaan sababtoo ah awood la'aanta inay u adkeystaan ​​​​xoogagga waaweyn ee centrifugal; si la mid ah, tuubooyinka microcentrifuge ee dhumucdiisuna waxay saameyn doonaan saamaynta PCR sababtoo ah wareejinta kulaylka oo gaabis ah iyo wareejinta kulaylka aan sinnayn.

3.sideed tuubbo

Sababo la xiriira culeyska shaqada ee tijaabada dufcadaha iyo shaqada aan habboonayn ee hal tubo, siddeed tuubbo oo saf ah ayaa la ikhtiraacay.Cotaus®PCR 8-strip tube waxay ka samaysan tahay polypropylene oo dibadda laga keeno, daboolka tuubadana waxa uu la mid yahay jidhka tuubada, kaas oo leh wax-qabad aad u wanaagsan. Isla mar ahaantaana, waxay leedahay la qabsi xooggan waxayna buuxin kartaa ujeedooyin tijaabo oo kala duwan.
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