Imaanshaha Cusub | IIBKA | Black Elisa Plates

Xaalado kala duwan oo ka mid ah cilmiga nolosha, go'aaminta wakhtiga, hufan iyo dhaqaalaha iyo qiyaasida antigens ama unugyada difaaca jirka ee ku jira muunada waa qayb muhiim ah.

Baaritaanka difaaca jirka ee Enzyme-linked (ELISA) wuxuu caddeeyey inuu yahay cilmi-baaris qiimo leh iyo qalab lagu ogaanayo cabbirka unugyada difaaca jirka ama antigens ee shaybaarka noolaha iyadoo la isku dhejinayo antigens ama unugyada difaaca jirka ee dusha sare ee side-waji adag, kaas oo u oggolaanaya enzyme ( Inta badan HRP-ku calaamadeysan falcelinta antigen-antibody ee dusha sare ee adag. Farsamadan waxaa loo isticmaali karaa in lagu ogaado antigens molecule waaweyn iyo unugyada difaaca gaarka ah, iwm. Waxay leedahay faa'iidooyinka degdegga ah, xasaasiga ah, fudud, iyo sidayaal waa sahlan tahay in la qiyaaso. Si kastaba ha ahaatee, dareenka iyo dhaqdhaqaaqa firfircoon ee ogaanshaha ELISA ayaa si weyn u xaddiday cilladaha farsamada nuugista iftiinka sababtoo ah saameynta weyn ee xaaladaha dibadda ee isbeddelka midabka ee xalka iyo qiyaasta hoose ee waxtarka leh ee qiimaha OD.

Tiknoolajiyada DELFIA ---- waa si fudud in enzyme HRP lagu beddelo chelate lanthanide (Eu, Sm, Tb, Dy) oo calaamadinaysa kahortaga ogaanshaha ee baaritaanka caadiga ah ee ELISA. Lanthanides-ka loo isticmaalo DELFIA waa nooc gaar ah oo walxaha fluorescent ah, kaas oo dhigaya baahida agabka tijaabada ah --- taarikada Elisa. Lanthanides waxay leeyihiin cimri dherer oo mikrose seconds ama xitaa millise seconds, taas oo marka lagu daro ogaanshaha waqtiga la xalliyo si weyn u yaraynaysaa faragelinta asalka ah ee autofluorescence, iyo isbeddelkooda ballaaran ee Strokes 'waxay si weyn u wanaajisaa dareenka baaritaanka.

Inta badan ELISA waxay doortaan saxanka calaamadaynta enzyme hufan sida side iyo weelka, laakiin iftiinka ka soo baxa falcelinta luminescence waa isotropic, iftiinka kaliya kama firdhi doono jihada toosan, laakiin sidoo kale wuu kala firdhiyaa jihada toosan, wuuna doonaa. si fudud u dhex mara farqiga u dhexeeya godadka kala duwan ee saxanka calaamadaynta enzyme hufan iyo darbiga godadka. Godadka deriska ayaa isdhexgala midba midka kale oo saameeya natiijooyinka tijaabada.

White Elisa Plates waxaa loo isticmaali karaa ogaanshaha iftiinka daciifka ah waxaana caadi ahaan loo isticmaalaa kiimikada guud iyo horumarinta midabka substrate (tusaale falanqaynta hidda-wadaha wariye luciferase dual).

Black White Plates Elisa waxay leeyihiin calaamad daciif ah marka loo eego taarikada calaamadaynta enzyme cad sababtoo ah nuugida iftiinka iyaga u gaar ah, waxaana guud ahaan loo isticmaalaa in lagu ogaado iftiinka xooggan, sida ogaanshaha fluorescence.

Faa'iidooyinka Cotaus®Elisa Plates

● Ku xidhid sare

Taarikada Cotaus®Elisa ee leh tuubo madow waxay ka samaysan yihiin walxo aan is-Fluorescent ahayn, oogada ayaa lagu daaweeyay si aad u wanaajiso awoodda isku xidhka borotiinka, taas oo gaari karta 500ng IgG/cm2, miisaanka molecular ee borotiinnada ugu muhiimsan waa> 10kD .

● Fluorescence asalka hooseeya wuxuu baabi'iyaa dhibaatooyinka ay keenaan falcelin aan gaar ahayn.

tubbada madow waxay baabi'in karaan xoogaa daciif ah oo soo dhexgala soo noqnoqda sababtoo ah waxay yeelan doontaa nuugid iftiinkeeda.

● Naqshad la go'i karo

Naqshad la go'i karo oo ah jir saxan cad oo ensaymes ah iyo slats ensaymes madow ayaa aad ugu habboon hawlgalka. Fiiro gaar ah u yeelo fal-dambiyeedka, ha ku qasbin inaad jebiso hal dhinac, haddii kale way fududahay in la jebiyo.

Kala soocidda alaabta

Faahfaahin dheeraad ah oo alaabta, fadlan xor u noqo inaad nala soo xiriirto