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Codsiyada Caadiga ah ee Taarikada ELISA

2024-06-12

Sida qalab tijaabo ah, qaabka asaasiga ah eesaxan ELISAwaa taxane microplates ah oo ka kooban walxo weji adag (sida borotiinada iyo unugyada difaaca jirka). Codsiga saxanka ELISA, muunadda la tijaabin doono waxay la falcelinaysaa unug gaar ah oo enzyme-ku suntan, ka dibna midab muuqda ayaa la soo saari doonaa iyadoo lagu darayo substrate matrix ah, iyo waxa ku jira ama hawsha molecule bartilmaameedka ah ayaa la qiyaasi doonaa. ama lagu qiimeeyay iyadoo la ogaanayo nuugista ama calaamada fluorescence. Kuwa soo socda waa codsiyada caadiga ah ee taarikada ELISA ee meelo kala duwan:

1. Falanqaynta tirada borotiinka: Taarikada ELISA waxaa loo isticmaali karaa in lagu cabbiro xoogga iyo dhaqdhaqaaqa borotiinnada ee shaybaarada noolaha sida serum iyo supernatants unug, iyagoo siinaya qalab awood leh oo lagu ogaanayo calaamadaha burooyinka, unugyada difaaca fayraska cagaarshowga, calaamadaha dhaawaca myocardial, iwm. iyo ka caawinta dhakhaatiirta baadhista hore iyo baadhista cudurrada.

2. Korjoogteynta Cytokine: Cilmi-baarista immunology,Taarikada ELISAwaxay qiyaasi kartaa heerarka cytokine ee dhaqanka unugyada sare ee unugyada ama dareeraha unugyada, taas oo gacan ka geysaneysa fahamka hababka bayoolojiga sida jawaabaha difaaca iyo bararka, waxayna muhiimad weyn u leedahay horumarinta daaweynta cusub iyo daawooyinka.

3. Cilmi-baarista Nucleic acid: Iyada oo loo marayo taarikada ELISA, saynisyahannadu waxay ogaan karaan oo ay falanqeyn karaan nuxurka iyo waxqabadka DNA ama RNA, waxay bixiyaan taageerada xogta cilmi-baarista bayoolajiga molecular sida muujinta hidda-wadaha iyo xakamaynta hidda-wadaha, iyo sii kor u qaadida horumarinta beeraha sida daaweynta hidda-socodka iyo tafatirka hidda-socodka.

4. Cilmi-baarista waxqabadka Enzyme: Taarikada ELISA waxay si sax ah u cabbiri kartaa dhaqdhaqaaqa enzyme, waxay ka caawisaa cilmi-baarayaasha inay fahmaan shaqada iyo habka nidaaminta enzymes ee noolaha, waxayna bixiyaan tixraacyo muhiim ah oo loogu talagalay cilmi-baarista injineernimada enzyme, injineernimada dheef-shiid kiimikaadka iyo qaybaha kale.

5. Cilmi-baarista is-dhexgalka molecular:Taarikada ELISAlooma isticmaali karo kaliya in lagu cabbiro waxa ku jira molecules, laakiin sidoo kale in la barto isdhexgalka ka dhexeeya molecules. Marka la isku daro tignoolajiyada sida hawada sare ee plasmon-ka iyo wareejinta tamarta resonance fluorescence, habka isku-xidhka iyo kala-goynta u dhexeeya molecules waa la kormeeri karaa wakhtiga dhabta ah, iyadoo la siinayo aragtiyo cusub iyo habab loogu talagalay naqshadaynta daroogada, isdhexgalka borotiinka iyo cilmi-baarista kale.

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