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Tusaha Tilmaamaha Pipette Shaybaarka Kala Duwan

2024-11-12

Maxay yihiin talooyinka pipette?

 

Talooyinka pipette waa qalab la tuuri karo oo loogu talagalay pipettes loo isticmaalo in si sax ah loogu wareejiyo dareeraha. Waxay ku yimaadaan cabbirro kala duwan, agabyo, iyo noocyo, sida kuwa caadiga ah, ku dheggan hoose, la sifeeyay, iyo talooyinka dhererka dheer.

 

Tilmaamaha Pipette waxay muhiim u yihiin shaybaarada sayniska waxaana si weyn looga isticmaalaa guud ahaan cilmiga nolosha, kimistariga, daawooyinka, bayotechnoolajiyada, iyo bayoolajiyada unugyada. Codsiyadooda kala duwan awgeed, hay'adaha sharciyeynta adduunka oo dhan waxay dejiyaan heerarka tayada si loo hubiyo saxnaanta iyo isku halaynta cilmi-baarista. Cotaus, oo ah shirkad caan ah oo soo saarta alaabada bayoolojiga ee Shiinaha, waxay soo saartaa talooyinka pipette tayo sare leh kuwaas oo ISO, CE, iyo FDA shahaadoeysan, hubinta isku halaynta iyo u hoggaansanaanta cilmi-baarista sayniska.

 

Maanta, aan sahaminno noocyada kala duwan ee talooyinka pipette, si aan u fahamno sifooyinkooda gaarka ah iyo codsiyada ku saabsan maaraynta dareeraha saxda ah.

 


Noocyada kala duwan ee talooyinka pipette

 

1. Talooyinka Pipette ee caadiga ah (Universal).

 

Talooyinka pipette-ka caadiga ah, oo sidoo kale loo yaqaano talooyinka caalamiga ah, ayaa badanaa laga sameeyaa tayo sare, polypropylene autoclavable. Waxay yihiin nooca ugu badan ee qalabka pipette ee loo isticmaalo shaybaarrada leh shuruudo waxqabad oo kala duwan kuwaas oo u dhexeeya saxnaanta sare ilaa dib-u-celinta dib-u-celinta ee dulqaadka weyn, loogu talagalay inay ku habboonaato noocyo badan oo noocyada pipette ah iyo moodooyinka, taas oo ka dhigaysa mid isku dhafan oo ku habboon dareeraha guud. maaraynta hawlaha. Lagu heli karo noocyada nadiifka ah iyo kuwa aan nadiifka ahayn labadaba iyadoo ku xiran baahida gaarka ah ee tijaabada.

 

Talooyin aan nadiif ahayn vs

 

Talooyin aan nadiif ahayn:Kuwaas waxa loo isticmaali karaa habsocodyada guud ee shaybaadhka halka madhalaysnimadu aanay muhiim ahayn. Waxay yihiin kuwo waxtar u leh hawlaha caadiga ah ama muunado aan xasaasi ahayn.

 

Talooyin Madhalays ahWaxay lagama maarmaan u yihiin codsiyada xasaasiga ah sida microbiology, bayoolaji molecular, iyo baaritaanka kiliinikada, maadaama horay loo nadiifiyay oo laga xaqiijiyay wasakhda sida RNase, DNase, iyo endotoxins iwm. Waxay u muuqan kartaa mid soo jiidasho leh in autoclave talooyinka aan nadiifka ahayn kuwa, laakiin autoclaving waxay baabi'in kartaa halista wasakheynta ay keenaan noolaha, tani macnaheedu maahan in talooyinka ay xor ka yihiin RNase iyo DNA.

 

Haddii aad u baahan tahay inaad samayso imtixaanno xasaasi ah halka tan looga baahan yahay, waa inaad doorataa talooyinka pipette ee nadiifka ah ee soo saaraha kaas oo caddayn kara in talooyinkoodu ka madax bannaan yihiin RNase iyo DNA.

 

Cotaustalooyinka caadiga ahku yimaadda cabbirro kala duwan (tusaale, 10 µL, 20 µL, 50 µL, 100 µL, 200 µL, 300 µL, 1000 µL).

 

2. Shaandhayso vs Tips-La'aan-Filter

 

Talooyinka sifeynta:Talooyinka la sifeeyay waxay ka kooban yihiin xannibaad yar, oo sida caadiga ah laga sameeyay walxaha hydrophobic, oo ku yaal gudaha caarada. Shaandhayntani waxay ka hortagtaa wasakhaynta u dhaxaysa shaybaarada iyo pipette-ka. Tilmaamaha shaandhaynta waxaa guud ahaan loogu talagalay in loo isticmaalo noocyo imtixaanno gaar ah. Tusaale ahaan, haddii muunadku yahay mid daxaloobay, kacsan, ama dabeecaddiisu aad u liidato, waxa ay wax u dhimi kartaa pipette-ka. Xaaladahan oo kale, talooyinka shaandhada ayaa lagula talinayaa.

 

Mar kasta oo aad damacdo dareere, aerosols ayaa ka soo baxaya caarada pipette. Haddii aadan isticmaalin talooyinka shaandhada, aerosols-yadani waxay u badan tahay inay wasakheeyaan pipettekaaga iyo shaybaarada xiga, oo saameeya natiijooyinkaaga tijaabada ah. Sidaa darteed, talooyinka shaandhada ayaa aad ugu kharash-ool ah tijaabooyinka saxda ah.

 

Talooyin aan sifeynin:Tilmaamaha aan shaandhaynta ahayn ayaa ah talooyinka ugu badan ee loo isticmaalo pipette-yada shaybaarada maadaama ay ka qaalisan yihiin talooyinka shaandhada. Waxay si fiican ugu habboon yihiin shaybaarrada aan u nugulayn wasakheynta oo aan laga yaabo inay waxyeeleeyaan pipette-ka. sida go'doominta DNA-da plasmidka, iyo rarista gelyada agarose, iyo kuwo kale. Si kastaba ha ahaatee, waxay ka maqan yihiin faa'iidooyinka ka-hortagga wasakhda ee talooyinka shaandhada, taasoo ka dhigaysa mid aan ku habboonayn tijaabooyinka muhiimka ah ama xasaasiga ah.

 

3. Haysashada Hoose vs Talooyinka Hayntaan Aan Hoose (Heerka)

 

Talooyinka pipette xajinta hooseeyawaxaa si gaar ah loogu talagalay in lagu yareeyo haynta dareeraha ee caarada dhexdeeda, hubinta in muunad wareejin badan oo sax ah oo hufan. Talooyinkani waxay ku habboon yihiin in lagu shaqeeyo dareere aan muuqan, dhegdheg leh, ama qaali ah halkaasoo la dhimo luminta muunadda ay muhiim tahay. Si kastaba ha ahaatee, way ka kharash badan yihiin talooyinka caadiga ah, talooyinkani waxay ku habboon yihiin ururinta muunadyada inta lagu jiro PCR, nadiifinta borotiinka, SDS-PAGE, cloning, DNA iyo codsiyada RNA iyo sidoo kale codsiyada falanqaynta borotiinka ee kala duwan.

 

4. Talooyin Gaaban vs. Dhererka Dheeraadka ah

 

Talooyin pipette gaabanwaxaa loogu talagalay in lagu isticmaalo taarikada ceelasha badan, sida 1536 ama 384-qaababka ceelasha, halkaasoo cabbirkooda yar ay si sax ah u beegsadaan ceelasha cidhiidhiga ah. Talooyinkani waxay sidoo kale wanaajiyaan ergonomics iyagoo u oggolaanaya tuubooyinka u dhow kursiga, yaraynta cududda cududda inta lagu jiro hawlaha soo noqnoqda. Ku habboon baadhista wax-soo-saarka sare iyo xoojinta raaxada shaybaarka.

 

Talooyinka pipette dhererka dheerway ka dheer yihiin talooyinka caadiga ah, iyagoo siinaya xakameyn wanaagsan oo wasakhaysan iyada oo u oggolaanaysa marinka hoose ee maraakiibta iyadoo la yareynayo xidhiidhka weelka. Talooyinkani waxay ku habboon yihiin in lagu isticmaalo qalabka shaybaadhka sida blocks ceelka qoto dheer iyo tuubooyinka microcentrifuge, hubinta in si sax ah loo dhaqo dareeraha meelaha ay adagtahay in la gaaro.

 

5. Talooyinka Pipette-ballaaran

 

Talooyinka pipette-ka ballaaranMuujinaya dhamaadka fog oo leh 70% ka weyn talooyinka caadiga ah, sifada ayaa si gaar ah faa'iido u leh baabi'inta xiiridda unugyada iyo iska caabinta socodka. Iyaga oo ka dhigaya kuwo ku habboon maaraynta shaybaarrada adag ee tuubada sida xadhkaha unugyada jilicsan, DNA-da genomic, hepatocytes, hybridomas, iyo dareerayaasha kale ee aadka u muuqda. Talooyinkani waxay yareeyaan xoogaga xiirida makaanikada, ka hortagga kala qaybsanaanta unugyada iyo hubinta jiritaanka unugyada sare iyo waxtarka dhejinta.


6. Talooyinka Pipette Robotic

 

Talooyinka pipette-kawaxaa loogu talagalay in lagu isticmaalo habab kala duwan oo dareere ah oo otomaatig ah iyo aaladaha tuubooyinka. Talooyinkani waxay xaqiijinayaan waafaqid summadaha (Hamilton, Beckman, Firfircoon, Tecaniwm) ee otomaatiga shaybaadhka, kor u qaadista saxnaanta iyo hufnaanta codsiyada sare-u-qaadista. Talooyinka robotka waxaa lagu maamulaa dulqaad adag marka loo eego talooyinka pipette-ga gacanta. Talooyinkan auto-robotic-ku waxay xaqiijinayaan saxnaanta sare, saxnaanta, iyo hufnaanta codsiyada sare ee qaybaha kala duwan, oo ay ku jiraan genomics, proteomics, iyo cilmi-baarista dawooyinka.

Tusaale:

Talooyinka pipette-ka-qabashadawaa tabo gaar ah oo loo isticmaalo hababka dhuumaha iswada ee loo qaabeeyey in lagu yareeyo kororka koronto-dhaliyaha inta lagu jiro maaraynta dareeraha. Talooyinkani waxay lama huraan u yihiin codsiyada halka faragelinta elektrostatic ay saamayn karto daacadnimada muunada ama saxnaanta hababka maaraynta dareeraha tooska ah.

 

7. Talooyinka Pipette Gaarka ah

 

Codsiyada qaarkood waxay u baahan yihiin naqshado caarada pipette gaar ah oo loogu talagalay hawlo gaar ah.


Tusaalooyinka:


Talooyin PCR:Talooyin loogu talagalay in lagu isticmaalo hababka falcelinta silsiladda polymerase (PCR) si looga hortago faddaraynta DNA-da la xoojiyay.
Talooyin Cryogenic:Si gaar ah loogu talagalay in lagu isticmaalo heerkul aad u hooseeya oo badiyaa ku yimaada dhismo adag, waara oo lagu qabto muunado la qaboojiyey.

 

Gabagabo

 

Doorashada talooyinka pipette waxay ku xiran tahay nooca tijaabada iyo nooca pipette ee loo isticmaalo. Haddi ay tahay maaraynta dareeraha guud, ka hortagga faddaraynta, ama la shaqaynta muunado jilicsan ama qaali ah, fahamka noocyada iyo sifooyinka talooyinka pipette waxay hubisaa wareejinta dareeraha saxda ah oo hufan ee shaybaarka. Had iyo jeer dooro caarada pipette ee ku habboon baahidaada cilmi-baarista gaarka ah si aad u hubiso natiijooyinka ugu wanaagsan iyo isku halaynta.

 

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