Hordhaca Elisa Plate

ELISA PLATE: In Enzyme Linked Immunosorbent Assay (ELISA), nadiifinta, xoojinta iyo saamiga antigens, unugyada difaaca jirka, unugyada difaaca ee calaamadeysan ama antigens ee ku lug leh falcelinta difaaca jirka; Nooca kaydinta, xoogga saarista iyo Xaaladaha sida xoogga ionic, qiimaha pH, heerkulka falcelinta iyo waqtiga ayaa door muhiim ah ka ciyaara. Intaa waxaa dheer, dusha sare ee polystyrene adag (Polystyrene) sida side ayaa sidoo kale door muhiim ah ka ciyaara xayeysiinta antigens, unugyada difaaca ama unugyada antigen-antibody.

Antigens, unugyada difaaca jirka iyo biomolecules kale ayaa lagu dhejiyaa dusha sare ee side iyada oo loo marayo habab kala duwan, oo ay ku jiraan adsorption dadban iyada oo loo marayo bonds hydrophobic, hydrophobic / ionic bonds, isku xidhka covalent iyada oo loo marayo hordhaca kooxaha kale ee firfircoon sida kooxaha amino iyo carbon, iyo iyada oo loo marayo wax ka beddelka dusha sare. . Isku xidhka hydrophilic galmada ka dib.

TheElisa Platewaxaa loo qaybin karaa 48-ceel iyo 96-ceel iyadoo loo eegayo tirada godadka. Midka caadiga ah ee la isticmaalo waa 96-well, kaas oo lagu dooranayo sida uu qabo akhristahaaga microplate.

Intaa waxaa dheer, waxaa jira kuwa la go'i karo iyo kuwa aan la goyn karin. Kuwa aan la go'i karin, dhejisyada sabuuradaha oo dhan waxay ku xiran yihiin wadajir. Ka dib, kuwa la go'i karo, sariiraha sabuuradaha ayaa la kala soocaa, iyo looxyada kala go'an Waxaa jira 12-dalool iyo 8-god. Guud ahaan, taariko ku suntan enzyme-ka la go'i karo ayaa si caadi ah loo isticmaalaa maalmahan. Haddii aad hore u soo iibsatay qaar ka mid ah taarikada noocaan ah, waxa aad hadda iibsan kartaa qaar ka mid ah xariijimaha ku calaamadsan enzyme-ka.

Inkastoo microplates-ka ay sameeyeen soosaarayaasha kala duwan ay u egyihiin isku mid guud ahaan, faahfaahinta yaryar qaarkood way ka duwanaan doonaan, sida qaab-dhismeedka, iwm. Tani waxay badanaa u baahan tahay in loo isticmaalo akhristayaasha microplate kala duwan. Sidaa darteed, marka aad isticmaalayso Marka aad dooranayso inaad iibsato akhristaha microplate, waa inaad sidoo kale tixgelisaa sida uu u eg yahay akhristahaaga microplate. Laakiin guud ahaan waa la waafajiyay, kaliya qaar ayaa ka duwanaan doona. Sababtoo ah walxaha saxanka enzyme guud ahaan waa polystyrene (PS), iyo polystyrene waxay leedahay xasillooni kiimiko oo liidata waxaana lagu milmi karaa noocyo kala duwan oo dareere organic ah (sida hydrocarbons caraf udgoon, hydrocarbons halogenated, iwm.), waxaana lagu daxaliyay asiidh xooggan. iyo alkalis. , oo aan u adkaysan karin dufan, oo si sahlan loo beddelo midabka ka dib marka lagu soo bandhigo iftiinka ultraviolet, markaa hubi inaad fiiro gaar ah u yeelato markaad isticmaalaysoElisa Plate.